Genetically Engineered Immunomodulatory Streptococcus thermophilus Strains Producing Antioxidant Enzymes Exhibit Enhanced Anti-Inflammatory Activities

• DOI: 10.1128/aem.03296-13
• Publication Date: 2013-11-16T05:55:13Z
• Authors: Silvina del Carmen; Alejandra de More...; Rebeca Martin; Florian Chain; Philippe Langella; Luis G. Bermúdez-...; Jean Guy LeBlanc
• Keywords: LACTOBACILLUS-CASEI; 570; INTESTINAL INFLAMMATION; [SDV]Life Sciences [q-bio]; INFLAMMATORY-BOWEL-DISEASE;LACTIC-ACBACTERIA;SUPEROXIDE-DISMUTASE;INTESTINAL INFLAMMATION;LACTOBACILLUS-CASEI;OXIDATIVE STRESS;MOUSE MODEL;DSS-COLITIS;IN-VITRO;MICE; 610; Antioxidants; DSS-COLITIS; Anti-Inflammatory; Mice; 03 medical and health sciences; https://purl.org/becyt/ford/2.6; LACTIC-ACBACTERIA; SUPEROXIDE-DISMUTASE; Animals; Immunologic Factors; Streptococcus thermophilus; https://purl.org/becyt/ford/2; OXIDATIVE STRESS; Cells, Cultured; Inflammation; 0303 health sciences; Superoxide Dismutase; MOUSE MODEL; IN-VITRO; Catalase; Colitis; MICE; Disease Models, Animal; Metabolic Engineering; Trinitrobenzenesulfonic Acid; Leukocytes, Mononuclear; Cytokines; Streptoccoccus Thermophilus; INFLAMMATORY-BOWEL-DISEASE

ABSTRACT The aims of this study were to develop strains lactic acid bacteria (LAB) having both immunomodulatory and antioxidant properties evaluate their anti-inflammatory effects in vitro , different cellular models, vivo a mouse model colitis. Different Lactobacillus delbrueckii subsp. bulgaricus Streptococcus thermophilus cocultured with primary cultures mononuclear cells. Analysis the pro- cytokines secreted by these cells after coincubation candidate revealed that L. CRL 864 S. 807 display highest profiles . Moreover, results confirmed determination cytokine large intestine samples mice fed strains. was then transformed two plasmids harboring genes encoding catalase (CAT) or superoxide dismutase (SOD) enzymes, recombinant streptococci evaluated colitis induced trinitrobenzenesulfonic (TNBS). Our showed decrease weight loss, lower liver microbial translocation, macroscopic microscopic damage scores, modulation production intestines treated either CAT- SOD-producing compared those wild-type strain control without any treatment. Furthermore, greatest activity observed receiving mixture streptococci. addition did not improve beneficial effects. These findings show genetically engineering bacterium (e.g., 807) intrinsic introducing gene expressing an enzyme enhances its activities.