Multistrain Probiotic Modulation of Intestinal Epithelial Cells' Immune Response to a Double-Stranded RNA Ligand, Poly(I·C)

• DOI: 10.1128/aem.03411-13
• Publication Date: 2013-12-28T03:28:48Z
• Authors: Chad MacPherson; Julie Audy; Olivier Mathieu; Thomas A. Tompkins
• Keywords: TLR3; Lactobacillus casei

ABSTRACT A commercially available product containing three probiotic bacterial strains ( Lactobacillus helveticus R0052, Bifidobacterium longum subsp. infantis R0033, and bifidum R0071) was previously shown in animal trials to modulate both T H 1 2 immune responses. Clinical studies on this combination of bacteria have also positive health effects against seasonal winter diseases rotavirus infection. The goal study use a well-established vitro intestinal epithelial (HT-29) cell model that has been constitutively express double-stranded RNA (dsRNA) sensors (Toll-like receptor 3 [TLR3], retinoic acid-inducible gene I, melanoma differentiation-associated 5, dsRNA-activated protein kinase). By using the HT-29 model, we wanted evaluate whether or not had capacity host response dsRNA ligand, poly(I·C). Using custom-designed, two-color expression microarray targeting genes human system, investigated cells challenged with poly(I·C) presence absence bacteria. We observed major impact attenuating connected proinflammatory antiviral innate responses compared obtained by poly(I·C)-only challenge. Major pathways through which multistrain may be eliciting its immune-modulatory effect include TLR3 domain-containing adapter-inducing beta interferon (TRIF), mitogen-activated kinase, NF-κB signaling pathways. Such useful for selecting potential biomarkers design future clinical trials.