Simultaneous Nitrite-Dependent Anaerobic Methane and Ammonium Oxidation Processes

• DOI: 10.1128/aem.05539-11
• Publication Date: 2011-08-13T02:32:37Z
• Authors: Francisca A. Luesken; Jaime Sánchez; Theo A. van Alen; Janeth Sanabria; Huub J. M. Op den...; Mike S. M. Jetten; Boran Kartal
• Keywords: Anammox; Candidatus; Library; Enrichment culture

ABSTRACT Nitrite-dependent anaerobic oxidation of methane (n-damo) and ammonium (anammox) are two recently discovered processes in the nitrogen cycle that catalyzed by n-damo bacteria, including “ Candidatus Methylomirabilis oxyfera,” anammox respectively. The feasibility coculturing bacteria is important for implementation wastewater treatment systems contain substantial amounts both ammonium. Here we tested this possible coexistence experimentally. To obtain such a coculture, was fed to stable enrichment culture still contained some residual bacteria. supplied reactor consumed rapidly could be gradually increased from 1 20 mM/day. enriched coculture monitored fluorescence situ hybridization 16S rRNA pmoA gene clone libraries activity measurements. After 161 days, with about equal established converted nitrite at rate 0.1 kg-N/m 3 /day (17.2 mmol day −1 ). This indicated application removal may feasible near future.