Production and characterization of aflatoxin B2a antiserum

• DOI: 10.1128/aem.41.2.478-482.1981
• Publication Date: 2020-01-01T03:07:53Z
• Authors: P K Gaur; H P Lau; J J Pestka; F S Chu
• Keywords: Bovine serum albumin; Horseradish peroxidase; Serum Albumin

The specificity and sensitivity of antiserum elicited from rabbits against aflatoxin B2a-bovine serum albumin conjugates were characterized with a radioimmunoassay (RIA) an enzyme-linked immunosorbent assay (ELISA). Aflatoxin B1 was first converted to B2a then conjugated bovine horseradish peroxidase by reductive alkylation method. developed in New Zealand white multiple-site injection the conjugate. Antibody titers determined both RIA ELISA. Competitive RIAs various analogs indicated that most reactive slightly cross-reactive aflatoxins B2a, B2, M1. ELISAs showed be equally specific for B12 less B2 relative sensitivities ELISA quantitation 100 10 pg per assay, respectively.