Cellular Location and Some Properties of Proteolytic Enzymes of Rumen Bacteria

• DOI: 10.1128/aem.43.5.1026-1033.1982
• Publication Date: 2020-01-01T03:16:45Z
• Authors: Jan Kopecny; R. John Wallace
• Keywords: Proteolytic enzymes; Cell envelope; Digestion

Several physical and chemical techniques were used to extract, identify the location of, proteolytic enzymes associated with mixed rumen bacteria. Most activity was removable by gentle methods such as shaking brief blending, without cell disruption, indicating that it coat capsular material. Proteases present also in envelope, corresponding inner membrane fraction of gram-negative bacteria, intracellularly detergent French press treatment. Temperature pH profiles obtained for enzymes, likely be most important digestion food protein. Inhibition studies indicated these proteases, those whole bacterial from fluid, predominantly cysteine protease type.