Rumen bacterial and fungal degradation of Digitaria pentzii grown with or without sulfur

• DOI: 10.1128/aem.46.3.738-748.1983
• Publication Date: 2020-01-01T03:28:08Z
• Authors: D E Akin; G L Gordon; J P Hogan
• Keywords: Digitaria

Sheep fed the forage Digitaria pentzii fertilized with sulfur were compared those unfertilized for rumen microbial population involved fiber degradation. No differences detected in bacterial as determined by anaerobic cultures on a habitat-simulating medium, xylan, or pectin, 35S labeling techniques protein, transmission electron microscopic studies of bacterium-fiber interactions. Rumen volume and water flow from not different sheep each forages. fungi prevalent sulfur-fertilized D. shown sporangia adhering to colonies developing zoospores roll tubes cellobiose plus streptomycin penicillin. Fungi absent extremely small numbers forage. Nylon bag digestibility showed that preferentially colonized lignified cells blade sclerenchyma 6 h caused extensive degradation 24 h. In absence bacteria vitro studies, hyphal development occurred; other tissues blades (i.e., mestome sheath xylem) attacked, resulting residue partially degraded weakened cell walls. Nonlignified also degraded. Breaking force tests leaf incubated penicillin fluid within nylon bags such at least twice fragile dry matter loss fungi, actively growing bacteria, could remove about 62% material. The response afforded useful vivo test study role these microbes Our data establish can be significant degraders further unique them attacking weakening lignocellulosic tissues. more residues attack explain greater intake consistently observed eating