Detection of coliform bacteria in water by polymerase chain reaction and gene probes

• DOI: 10.1128/aem.56.2.307-314.1990
• Publication Date: 2020-01-01T04:21:51Z
• Authors: A K Bej; R J Steffan; J DiCesare; L Haff; R M Atlas
• Keywords: Primer (cosmetics); Indicator bacteria

Polymerase chain reaction (PCR) amplification and gene probe detection of regions two genes, lacZ lamB, were tested for their abilities to detect coliform bacteria. Amplification a segment the coding region Escherichia coli by using PCR primer annealing temperature 50 degrees C detected E. other bacteria (including Shigella spp.) but not Salmonella spp. noncoliform lamB selectively radiolabeled probes as little 1 10 fg genomic DNA few 5 viable cells in 100 ml water. provides basis method indicators fecal contamination water, particular permits enteric pathogens (Salmonella with necessary specificity sensitivity monitoring bacteriological quality water so ensure safety supplies.