Characterization of biphenyl catabolic genes of gram-positive polychlorinated biphenyl degrader Rhodococcus sp. strain RHA1

• DOI: 10.1128/aem.61.6.2079-2085.1995
• Publication Date: 2020-01-06T18:08:56Z
• Authors: E Masai; A Yamada; J M Healy; T Hatta; K Kimbara; M Fukuda; K Yano
• Keywords: Pseudomonas putida; Homology; Dioxygenase

Rhodococcus sp. strain RHA1 is a gram-positive polychlorinated biphenyl (PCB) degrader which can degrade 10 ppm of PCB48 (equivalent to Aroclor1248), including tri-, tetra-, and pentachlorobiphenyls, in few days. We isolated the 7.6-kb EcoRI-BamHI fragment carrying catabolic genes determined their nucleotide sequence. On basis deduced amino acid sequence homology, we identified six bph genes, bphA1A2A3A4, bphB, bphC, that are responsible for initial three steps degradation. The order bphA1A2A3A4-bphC-bphB. This gene differs from other PCB degraders reported previously. sequences have higher degree homology with tod Pseudomonas putida F1 (49 79%) than strains KF707 KKS102 (30 65%). In Escherichia coli, bphA activity was not observed even when expression vectors were used. activities bphB however, confirmed by observing transformation meta-cleavage compound aid benzene dioxygenase complemented (S. Irie, S. Doi, T. Yorifuji, M. Takagi, K. Yano, J. Bacteriol. 169:5174-5179, 1987). expected products cloned except bphA3, E. coli an vitro transcription-translation system. Insertion mutations bphA1 bphC constructed replacement fragments.(ABSTRACT TRUNCATED AT 250 WORDS)