Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose (glucose) isomerase

• DOI: 10.1128/aem.62.12.4648-4651.1996
• Publication Date: 2020-01-06T18:14:50Z
• Authors: M Walfridsson; X Bao; M Anderlund; G Lilius; L Bülow; B Hahn-Hägerdal
• Keywords: Xylose isomerase; Thermus thermophilus

The Thermus thermophilus xylA gene encoding xylose (glucose) isomerase was cloned and expressed in Saccharomyces cerevisiae under the control of yeast PGK1 promoter. recombinant showed highest activity at 85 degrees C with a specific 1.0 U mg-1. A new functional metabolic pathway S. ethanol formation during oxygen-limited fermentation demonstrated. Xylitol acetic acid were also formed fermentation.