Characterization of Colletotrichum gloeosporioides isolates from avocado and almond fruits with molecular and pathogenicity tests

• DOI: 10.1128/aem.62.3.1014-1020.1996
• Publication Date: 2020-01-06T18:15:57Z
• Authors: S Freeman; T Katan; E Shabi
• Keywords: HaeIII; genomic DNA; Colletotrichum

One hundred twenty isolates of Colletotrichum gloeosporioides from avocado (6 U.S. and 57 Israeli isolates) almond (57 fruits were compared by various molecular methods a pathogenicity assay in order to determine the genetic diversity host specificity between among different populations. DNA eight additional anthracnose also compared. PCR amplification genomic with four primers produced uniform banding patterns for all geographic locations Israel. DNAs distinct isolates. In contrast, Israel United States more diverse, numerous arbitrarily primed-PCR phenotypes being observed. HaeIII digestion A+T-rich distinguished Southern hybridization repetitive nuclear-DNA element GcpR1 PstI-digested revealed no polymorphic fragments isolates, whereas observed Amplification subsequent restriction enzyme internal transcribed spacer 4 5 regions small large nuclear subunits encoding rRNA failed distinguish C. diverse range. artificial inoculations, lesions on fruits, infected both at lower rate.