Comparison of Gas Chromatography and Mineralization Experiments for Measuring Loss of Selected Polychlorinated Biphenyl Congeners in Cultures of White Rot Fungi

• DOI: 10.1128/aem.64.6.2020-2025.1998
• Publication Date: 2019-12-19T19:36:02Z
• Authors: Lee A. Beaudette; Stephen Davies; Phillip M. Fedorak; Owen P. Ward; Michael A. Pickard
• Keywords: Phanerochaete; Manganese peroxidase; Congener; Chrysosporium; Lignin peroxidase; Polychlorinated biphenyl; Biphenyl

ABSTRACT Two methods were used to compare the biodegradation of six polychlorinated biphenyl (PCB) congeners by 12 white rot fungi. Four fungi found be more active than Phanerochaete chrysosporium ATCC 24725. Biodegradation following was monitored gas chromatography: 2,3-dichlorobiphenyl, 4,4′-dichlorobiphenyl, 2,4′,5-trichlorobiphenyl (2,4′,5-TCB), 2,2′,4,4′-tetrachlorobiphenyl, 2,2′,5,5′-tetrachlorobiphenyl, and 2,2′,4,4′,5,5′-hexachlorobiphenyl. The congener tested for mineralization 2,4′,5-[U- 14 C]TCB. Culture supernatants also assayed lignin peroxidase manganese activities. Of tested, two strains Bjerkandera adusta (UAMH 8258 UAMH 7308), one strain Pleurotus ostreatus 7964), Trametes versicolor 8272 gave highest mineralization. P. 24725, a frequently in studies PCB degradation, lowest activities reported here. Low but detectable levels activity present culture supernatants, no correlation observed among any combination biodegradation, mineralization, or activity. With exception , loss ranged from 40 96%; however, these values varied due nonspecific binding fungal biomass glassware. Mineralization much lower, ≤11%, because it measures complete oxidation at least part molecule results consistent therefore reliable assessment biodegradation.