Distribution of Bifidobacterial Species in Human Intestinal Microflora Examined with 16S rRNA-Gene-Targeted Species-Specific Primers

• DOI: 10.1128/aem.65.10.4506-4512.1999
• Publication Date: 2019-12-19T19:42:50Z
• Authors: Takahiro Matsuki; Koichi Watanabe; Ryuichiro Tanaka; Masafumi Fukuda; Hiroshi Oyaizu
• Keywords: Bifidobacterium longum; Human feces; Actinomycetaceae; Bifidobacterium breve; Bifidobacterium bifidum; Human gastrointestinal tract

ABSTRACT In order to clarify the distribution of bifidobacterial species in human intestinal tract, a 16S rRNA-gene-targeted species-specific PCR technique was developed and used with DNAs extracted from fecal samples obtained 48 healthy adults 27 breast-fed infants. To cover all that have been isolated identified primers for Bifidobacterium longum , B. infantis dentium gallicum were adolescentis angulatum bifidum breve catenulatum group ( pseudocatenulatum ) previous study (T. Matsuki, K. Watanabe, R. Tanaka, H. Oyaizu, FEMS Microbiol. Lett. 167:113–121, 1998). The specificity nine confirmed by PCR, method found be useful means identifying strains feces. results an examination showed most commonly taxon (detected 44 [92%]), followed adult flora frequently tracts present demonstrated qualitative detection feces can accomplished rapidly accurately.