Biological and Molecular Detection of Toxic Lipodepsipeptide-Producing Pseudomonas syringae Strains and PCR Identification in Plants

• DOI: 10.1128/aem.65.5.1904-1909.1999
• Publication Date: 2019-12-19T19:50:38Z
• Authors: Alain Bultreys; Isabelle Gheysen
• Keywords: Pseudomonas syringae; Pseudomonadales

ABSTRACT Toxin-based identification procedures are useful for differentiating Pseudomonas syringae pathovars. A biological test on peptone-glucose-NaCl agar in which the yeast Rhodotorula pilimanae was used proved to be more reliable detecting lipodepsipeptide-producing strains of P. than usual potato dextrose Geotrichum candidum is used. PCR performed with primers designed amplify a 1,040-bp fragment coding sequence syrD gene, assumed involved syringomycin and syringopeptin secretion, efficiently detected gene pathovars that produce lipodepsipeptides. Comparable results were obtained both tests syringomycin-producing organisms pv. syringae, atrofaciens, aptata, but failed syringotoxin-producing fuscovaginae strain. The specificity verified by obtaining negative related or species do not toxic repeatedly liquid medium inoculated diseased vegetative tissue assayed test. Our procedure also adapted detect morsprunorum cfl gene-based