Characterization of Methylglyoxal Synthase from Clostridium acetobutylicum ATCC 824 and Its Use in the Formation of 1,2-Propanediol

• DOI: 10.1128/aem.65.7.3244-3247.1999
• Publication Date: 2019-12-19T19:53:24Z
• Authors: Ke-xue Huang; Frederick B. Rudolph; George N. Bennett
• Keywords: Clostridium acetobutylicum; Dihydroxyacetone phosphate

ABSTRACT A gene encoding a putative 150-amino-acid methylglyoxal synthase was identified in Clostridium acetobutylicum ATCC 824. The enzyme overexpressed Escherichia coli and purified. Methylglyoxal has native molecular mass of 60 kDa an optimum pH 7.5. K m V max values for the substrate dihydroxyacetone phosphate were 0.53 mM 1.56 mmol min −1 μg , respectively. When E. glycerol dehydrogenase coexpressed with BL21(DE3), 3.9 1,2-propanediol produced.