The Functional ccpA Gene Is Required for Carbon Catabolite Repression in Lactobacillus plantarum

• DOI: 10.1128/aem.67.7.2903-2907.2001
• Publication Date: 2002-07-27T10:03:59Z
• Authors: Lidia Muscariello; Rosangela Marasco; Maurilio De Felice; Margherita Sacco
• Keywords: CCPA; Transcription

ABSTRACT We report the characterization of ccpA gene Lactobacillus plantarum , coding for catabolite control protein A. The is linked to pepQ gene, encoding a proline peptidase, in order ccpA-pepQ with two genes transcribed tandem from same strand as distinct transcriptional units. Two transcription start sites corresponding functional promoters were found, expression upstream promoter being autogenously regulated through catabolite-responsive element ( cre ) sequence overlapping +1 site. During growth on ribose, showed maximal expression, while glucose led downstream promoter. In mutant strain, was mainly both repressing and non conditions. Expression enzyme activities, β-glucosidase β-galactosidase, relieved carbon repression strain. vivo footprinting analysis catabolite-controlled bglH regulatory region strain loss protection under