Identification of Deoxynivalenol- and Nivalenol-Producing Chemotypes of Gibberella zeae by Using PCR

• DOI: 10.1128/aem.67.7.2966-2972.2001
• Publication Date: 2002-07-27T10:00:58Z
• Authors: Theresa Lee; Dae-Woong Oh; Hye-Seon Kim; Jungkwan Lee; Yong-Ho Kim; Sung-Hwan Yun; Yin-Won Lee
• Keywords: Gibberella zeae; Chemotype; Gibberella; Gene cluster; Primer (cosmetics)

ABSTRACT Gibberella zeae , a major cause of cereal scab, may be divided into two chemotypes based on production the trichothecenes deoxynivalenol (DON) and nivalenol (NIV). We cloned sequenced gene cluster for trichothecene biosynthesis from each chemotype. G. H-11 is DON producer isolated corn, 88-1 NIV barley. 23-kb 26-kb 88-1, along with unlinked Tri101 genes. Each contained 10 Tri homologues in same order transcriptional directions as those Fusarium sporotrichioides . Between all except Tri7 were conserved, identities ranging 88 to 98% 82 99% at nucleotide amino acid levels, respectively. The sequences only 80% identical level. aligned genes found that open reading frame carried several mutations an insertion containing copies 11-bp tandem repeat. neither repeat nor mutations. assayed 100 isolates both by PCR amplification primer pair derived could differentiate polyacrylamide gel electrophoresis. PCR-based method developed this study should provide simple reliable diagnostic tool differentiating