Wax Ester Production from n -Alkanes by Acinetobacter sp. Strain M-1: Ultrastructure of Cellular Inclusions and Role of Acyl Coenzyme A Reductase

• DOI: 10.1128/aem.68.3.1192-1195.2002
• Publication Date: 2002-07-27T10:09:29Z
• Authors: Takeru Ishige; Akio Tani; Keiji Takabe; Kazunori Kawasaki; Yasuyoshi Sakai; Nobuo Kato
• Keywords: Strain (injury); Alkane

ABSTRACT Acinetobacter sp. strain M-1 accumulated a large amount of wax esters from an n -alkane under nitrogen-limiting conditions. Under the optimized conditions with -hexadecane as substrate, hexadecyl hexadecanoate in cells reached 0.17 g/g (dry weight). Electron microscopic analysis revealed that multilayered disk-shaped intracellular inclusions were formed concomitant ester formation. The contribution acyl-CoA reductase to synthesis was evaluated by gene disruption analysis.