Molecular Method To Assess the Diversity of Burkholderia Species in Environmental Samples

• DOI: 10.1128/aem.68.4.1595-1603.2002
• Publication Date: 2002-07-27T09:59:40Z
• Authors: Joana Falcão Salles; Francisco Adriano...; Jan Dirk van Elsas
• Keywords: Amplicon

ABSTRACT In spite of the importance many members genus Burkholderia in soil microbial community, no direct method to assess diversity this has been developed so far. The aim work was development DNA-based PCR-denaturing gradient gel electrophoresis (DGGE), a powerful tool for studying communities, detection and analysis samples. Primers specific were based on 16S rRNA gene sequence evaluated PCRs performed with genomic DNAs from non- species as templates. primer system used exhibited good specificity sensitivity majority established . DGGE analyses PCR products obtained showed that there sufficient differences migration behavior distinguish 14 tested. Sequence amplicons generated DNA exclusively revealed sequences affiliated species, demonstrating PCR-DGGE is suitable natural settings. A communities two grassland plots mainly between bulk rhizosphere samples; latter samples produced more complex patterns.