Diversity of Bacteria Associated with Natural AphidPopulations

• DOI: 10.1128/aem.69.12.7216-7223.2003
• Publication Date: 2003-12-05T20:42:58Z
• Authors: S. Haynes; A. C. Darby; T. J. Daniell; G. Webster; F. J. F. van Veen; H.C.J. Godfray; J. I. Prosser; A. E. Douglas
• Keywords: Acyrthosiphon pisum; Buchnera; Candidatus; Symbiotic bacteria; Aphis

ABSTRACT The bacterial communities of aphids were investigated by terminal restriction fragment length polymorphism and denaturing gradient gel electrophoresis analysis 16S rRNA gene fragments generated PCR with general eubacterial primers. By both methods, theγ -proteobacterium Buchnera was detected in laboratory cultures six parthenogenetic lines the pea aphid Acyrthosiphon pisum one line black bean Aphis fabae , or more four previously described taxa also all except A. . These latter bacteria, collectively known as secondary symbionts accessory comprised three γ-proteobacteria (R-type [PASS], T-type [PABS], U-type [PAUS]) a rickettsia (S-type [PAR]). Complementary from natural populations species ( [ n = 74], Amphorophora rubi 109], sarothamni 42], Microlophium carnosum 101]) single geographical location revealed up to but no other taxa, each aphid. prevalence varied significantly among not sampling month (between June August 2000). results indicate that are distributed across multiple species, although variable prevalence, culture does generally result shift community aphids. Both transmission patterns bacteria between individual their impact on fitness suggested influence populations.