Comparison of Method 1623 and Cell Culture-PCR for Detection of Cryptosporidium spp. in Source Waters

• DOI: 10.1128/aem.69.2.971-979.2003
• Publication Date: 2003-02-06T00:43:15Z
• Authors: Mark W. LeChevallier; George D. Di Giov...; Jennifer L. Clancy; Zia Bukhari; Shan Bukhari; Jeffrey S. Rosen; Jose Sobrinho; Michelle M. Frey
• Keywords: Cryptosporidium parvum

ABSTRACT Analysis of Cryptosporidium occurrence in six watersheds by method 1623 and the integrated cell culture-PCR (CC-PCR) technique provided an opportunity to evaluate these two methods. The average recovery efficiencies were 58.5% for CC-PCR 72% 1623, but values not significantly different ( P = 0.06). oocysts detected 60 593 samples (10.1%) 1623. Infectious 22 560 (3.9%) technique. There was 87% agreement between total numbers positive as determined four sites. other sites had 16.3 24% correspondence all watersheds. Overall, approximately 37% immunofluorescence viable infectious. DNA sequence analysis parvum isolates showed presence both bovine human genotypes. More than 90% C. identified having or bovine-like genotype. estimates concentrations infectious resulting daily annual risks infection compared well results suggest that most surface water systems would require, on average, a 3-log reduction source levels meet potable goals.