Characterization of Lactobacillus coryniformis DSM 20001 T Surface Protein Cpf Mediating Coaggregation with and Aggregation among Pathogens

• DOI: 10.1128/aem.70.12.7078-7085.2004
• Publication Date: 2004-12-01T20:18:56Z
• Authors: Martina Schachtsiek; Walter P. Hammes; Christian Hertel
• Keywords: Strain (injury)

Phenotypic characterization of aggregation phenotypes Lactobacillus coryniformis revealed that strain DSM 20001T coaggregated with Escherichia coli K88, Campylobacter coli, and jejuni but not other human pathogens. In addition, cells these pathogens aggregated in the presence spent culture supernatant (SCS) 20001T. Cells E. K88 remained viable coaggregates aggregates for up to 24 h. Both coaggregation (co/aggregation) occurred at pH 3.5 7.5 was sensitive heat (85 degrees C 15 min) proteinase K. The co/aggregation-promoting factor (Cpf) purified, gene identified by PCR degenerate primers derived from internal amino acid sequences. cpf encoded a 19.9-kDa preprotein sec-dependent leader an isoelectric point 4.4. sequence had no significant similarity proteins known functions. Northern analysis only major transcription promoter also preceding insertion element, ISLco1 belonging IS3 family. Recombinant Cpf produced mediated comparable obtained purified or SCS could be removed treatment 5 M LiCl subsequently reattached cell surface using recombinant Cpf, which resulted restoration co/aggregation property. These results together those suggest is novel protein L. mediates some