Distribution of Giardia duodenalis Genotypes and Subgenotypes in Raw Urban Wastewater in Milwaukee, Wisconsin

• DOI: 10.1128/aem.70.6.3776-3780.2004
• Publication Date: 2004-06-07T23:03:20Z
• Authors: Irshad M. Sulaiman; Jianlin Jiang; Ajaib Singh; Lihua Xiao
• Keywords: Giardia; Assemblage (archaeology); Giardia lamblia

ABSTRACT Giardia cysts in 131 raw wastewater samples from Milwaukee, Wis., were genotyped by sequence analysis of the triosephosphate isomerase gene which showed presence two distinct genotypes (assemblages A and B) duodenalis . Of samples, 111 belonged to assemblage A, remaining B. high degree genetic polymorphism was evident within B cluster, with 10 subgenotypes identified, eight have not been reported before.