Cell Density-Dependent Gene Contributes to Efficient Seed Colonization by Pseudomonas putida KT2440

• DOI: 10.1128/aem.70.9.5190-5198.2004
• Publication Date: 2004-09-02T20:15:34Z
• Authors: Manuel Espinosa-U...; Juan-Luis Ramos
• Keywords: Pseudomonas putida; rpoS; Salmonella enterica

ABSTRACT We have characterized the expression pattern of a gene, ddcA , involved in initial colonization corn seeds by Pseudomonas putida KT2440. The gene codes for putative membrane polypeptide belonging to family conserved proteins unknown function. Members this are widespread among prokaryotes and include products Salmonella enterica serovar Typhimurium expressed during invasion macrophages psiE an Escherichia coli phosphate starvation-inducible gene. Although its specific role is undetermined, presence multicopy restored seed adhesion capacity KT2440 mutant. Expression growth phase regulated, being maximal at beginning stationary phase. It independent RpoS, nutrient depletion, or starvation, it not result changes medium pH growth. directly dependent on cell density, also stimulated addition conditioned exudates. This first evidence suggesting existence quorum-sensing system P. potential implication such signaling process bacterium discussed.