Role of Hexose Transport in Control of Glycolytic Flux in Saccharomyces cerevisiae

• DOI: 10.1128/aem.70.9.5323-5330.2004
• Publication Date: 2004-09-02T20:15:34Z
• Authors: Karin Elbing; Christer Larsson; Roslyn M. Bill; Eva Albers; Jacky L. Snoep; Eckhard Boles; Stefan Hohmann; Lena Gustafsson
• Keywords: Hexose; Chemostat

ABSTRACT The yeast Saccharomyces cerevisiae predominantly ferments glucose to ethanol at high external concentrations, irrespective of the presence oxygen. In contrast, low concentrations and in oxygen, as a glucose-limited chemostat, no is produced. importance concentration suggests central role for affinity maximal transport rates yeast's transporters control production. Here we present series strains producing functional chimeras between hexose Hxt1 Hxt7, each which has distinct characteristics. display range decreasing glycolytic resulting proportional decrease Using these strains, show first time that levels, uptake capacity wild-type S. does not flux during exponential batch growth. our chimeric Hxt rate glycolysis degree. Strains whose mediated by will undoubtedly provide powerful tool with examine detail mechanism underlying switch fermentation respiration new tools industrial fermentations.