Evaluation of New Broth Media for Microdilution Antibiotic Susceptibility Testing of Lactobacilli, Pediococci, Lactococci, and Bifidobacteria

• DOI: 10.1128/aem.71.12.8982-8986.2005
• Publication Date: 2005-12-06T11:07:48Z
• Authors: Ingo Klare; Carola Konstabel; Sibylle Müller-B...; Rolf Reissbrodt; Geert Huys; Marc Vancanneyt; Jean Swings; Herman Goossens; Wolfgang Witte
• Keywords: 0301 basic medicine; Staphylococcus; Streptococcus; Microbial Sensitivity Tests; Anti-Bacterial Agents; Culture Media; 3. Good health; Lactobacillus; 03 medical and health sciences; Lactococcus; Escherichia coli; Bifidobacterium; Pediococcus; Enterococcus

ABSTRACT

Nine pure or mixed broth media were evaluated for their suitabilities to determine MICs in a microdilution test of 19 antibacterial agents for lactic acid bacteria (LAB) of the genera Lactobacillus , Pediococcus , Lactococcus , and Bifidobacterium . A mixed formulation of Iso-Sensitest broth (90%) and deMan-Rogosa-Sharpe broth (10%) with or without supplementation with l -cysteine, referred to as the LAB susceptibility test medium, provided the most optimal medium basis in terms of growth support of nonenterococcal LAB and correct indication of MICs of international control strains.