Influence of Dietary Zinc Oxide and Copper Sulfate on the Gastrointestinal Ecosystem in Newly Weaned Piglets

• DOI: 10.1128/aem.71.5.2267-2277.2005
• Publication Date: 2005-05-03T18:08:24Z
• Authors: Ole Højberg; Nuria Canibe; Hanne Damgaard Po...; Mette Skou Hedemann; Bent Borg Jensen
• Keywords: Cecum; Lactobacillus reuteri

ABSTRACT Dietary doses of 2,500 ppm ZnO-Zn reduced bacterial activity (ATP accumulation) in digesta from the gastrointestinal tracts newly weaned piglets compared to that animals receiving 100 ZnO-Zn. The amounts lactic acid bacteria (MRS counts) and lactobacilli (Rogosa were reduced, whereas coliforms (MacConkey enterococci (Slanetz counts, red colonies) more numerous high ZnO dose. Based on 16S rRNA gene sequencing, colonies MRS dominated by three phylotypes, tentatively identified as Lactobacillus amylovorus (OTU171), reuteri (OTU173), Streptococcus alactolyticus (OTU180). Rogosa plates two phylotypes only. Terminal restriction fragment length polymorphism analysis supported observations dominating low dose 175 CuSO 4 -Cu also counts stomach contents, but for these animals, numbers cecum colon. influence microbiota resembles working mechanism suggested some growth-promoting antibiotics, namely, suppression gram-positive commensals rather than potentially pathogenic gram-negative organisms. Reduced fermentation digestible nutrients proximal part tract may render energy available host animal contribute effect dietary doses. inhibited thus potential pathogens well, overall observed was limited ZnO.