Effects of Lactococcus lactis on Composition of Intestinal Microbiota: Role of Nisin

• DOI: 10.1128/aem.72.1.239-244.2006
• Publication Date: 2006-01-03T20:33:36Z
• Authors: Nete Bernbom; Tine Rask Licht; Carl-Henrik Brogren; Birthe Jelle; Anette H. Johansen; Iker Badiola; Finn K. Vogensen; Birgit Nørrung
• Keywords: Cecum

ABSTRACT This study examined the ability of (i) pure nisin, (ii) nisin-producing Lactococcus lactis strain CHCC5826, and (iii) non-nisin-producing L. CHCH2862 to affect composition intestinal microbiota human flora-associated rats. The presence both strains significantly increased number Bifidobacterium cells in fecal samples during first 8 days but decreased enterococci/streptococci duodenum, ileum, cecum, colon as detected by selective cultivation. No significant changes rat were observed after dosage with nisin. Pearson cluster analysis denaturing gradient gel electrophoresis profiles 16S rRNA genes present microbial population revealed that animals dosed either two different from control saline. However, nisin did not differ controls. concentrations estimated competitive enzyme-linked immunosorbent assay (ELISA) approximately 10-fold higher small intestine 200-fold feces than corresponding a biological assay. indicates was degraded or inactivated gastrointestinal tract, since fragments this bacteriocin are ELISA while an intact molecule is needed retain activity.