Genome Sequence of the Chemolithoautotrophic Nitrite-Oxidizing Bacterium Nitrobacter winogradskyi Nb-255

• DOI: 10.1128/aem.72.3.2050-2063.2006
• Publication Date: 2006-03-03T17:47:56Z
• Authors: Shawn R. Starkenburg; Patrick S. G. Chain; Luis A. Sayavedra...; Loren Hauser; Miriam L. Land; Frank W. Larimer; Stephanie A. Malf...; Martin G. Klotz; Peter J. Bottomley; Daniel J. Arp; William J. Hickey
• Keywords: Nitrobacter; Alphaproteobacteria

ABSTRACT The alphaproteobacterium Nitrobacter winogradskyi (ATCC 25391) is a gram-negative facultative chemolithoautotroph capable of extracting energy from the oxidation nitrite to nitrate. Sequencing and analysis its genome revealed single circular chromosome 3,402,093 bp encoding 3,143 predicted proteins. There were extensive similarities genes in two alphaproteobacteria, Bradyrhizobium japonicum USDA110 (1,300 genes) Rhodopseudomonas palustris CGA009 CG (815 genes). Genes pathways for known modes chemolithotrophic chemoorganotrophic growth identified. multiple enzymes involved anapleurotic reactions centered on C 2 4 metabolism, including glyoxylate bypass, annotated. inability N. grow 6 molecules consistent with sequence, which lacks complete Embden-Meyerhof Entner-Doudoroff pathways, active uptake sugars. Two gene copies oxidoreductase, type I ribulose-1,5-bisphosphate carboxylase/oxygenase, cytochrome c oxidase, homologs an aerobic-type carbon monoxide dehydrogenase present. Similarity oxidoreductases respiratory nitrate reductases was confirmed. Approximately 10% codes transport secretion, presence transporters various organic-nitrogen molecules. provides new insight into phylogenetic identity physiological capabilities nitrite-oxidizing bacteria. will serve as model study cellular molecular processes that control interaction other nitrogen-cycling processes.