Substance P augments tumor necrosis factor release in human monocyte-derived macrophages

• DOI: 10.1128/cdli.1.4.419-423.1994
• Publication Date: 2020-01-06T18:33:45Z
• Authors: H R Lee; W Z Ho; S D Douglas
• Keywords: Monokine; Monocyte

Substance P (SP) is an undecapeptide that has the amino sequence Arg-Pro-Lys-Pro-Gin-Gln-Phe-Phe-Gly-Leu-Met-NH2 and belongs to a family of structurally related peptides known as tachykinins, latter are widely distributed in central nervous system. SP involved biological activities cells immune system, including induction cytokines cells. We have investigated effects on constitutive and/or lipopolysaccharide (LPS)-induced expression tumor necrosis factor (TNF) cultured blood monocyte-derived macrophages (MDM). Cells vitro for 14 days were treated with at various concentrations (10(-10) 10(-6M) presence LPS before culture supernatants harvested. TNF bioactivity was measured L929 cell line MDM from 10 12 donors alone showed increased production. also interacted synergistic fashion upregulating production responders. The stimulatory effect inhibited by two antagonists, spantide ([D-Arg-1-D-Trp-7-D-Trp-7-D-Trp-9-leu-11]-SP) CP-96,345 (a nonpeptide antagonist receptor). In addition, anti polyclonal antibody blocked MDM, further indicating specificity these effects. These results demonstrate important regulator monokine human monocytes/macrophages.