Noninfectious Virus-Like Particle Antigen for Detection of Swine Vesicular Disease Virus Antibodies in Pigs by Enzyme-Linked Immunosorbent Assay

• DOI: 10.1128/cdli.12.8.922-929.2005
• Publication Date: 2005-08-05T22:13:00Z
• Authors: Young-Joon Ko; Kang-Seuk Choi; Jin-Ju Nah; David J. Paton; Jae-Ku Oem; Ginette Wilsden; Shien-Young Kang; Nam-In Jo; Joo-Ho Lee; Jae-Hong Kim; Hee-Woo Lee; Jong-Myeong Park
• Keywords: 0301 basic medicine; Insecta; Base Sequence; Swine; Molecular Sequence Data; Antibodies, Monoclonal; Enzyme-Linked Immunosorbent Assay; Antibodies, Viral; Sensitivity and Specificity; Recombinant Proteins; Cell Line; 3. Good health; 03 medical and health sciences; Swine Vesicular Disease; Animals; Antigens, Viral; Baculoviridae

ABSTRACT An inactivated SVDV antigen is used in current enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies to swine vesicular disease virus (SVDV). To develop a noninfectious recombinant alternative, we produced SVDV-like particles (VLPs) morphologically and antigenically resembling authentic by using dual baculovirus recombinant, which expresses simultaneously P1 3CD protein genes under different promoters. Antigenic differences between VLPs were not statistically significant results obtained with 5B7-ELISA kit, indicating that could be place ELISA kits. We developed blocking SVDV-specific neutralizing monoclonal antibody 3H10 (VLP-ELISA) serum pigs. The VLP-ELISA showed high specificity 99.9% when tested pig sera are negative neutralization ( n = 1,041). When 186) collected periodically from pigs 19) experimental infection each three strains SVDV, detected as early 3 days postinfection continued detect all infected until termination experiments (up 121 postinfection). This test performance was similar gold standard indicates highly specific sensitive method first report production diagnostic application SVDV. Further potential uses discussed.