Interleukin-12 Is the Optimum Cytokine To Expand Human Th17 Cells In Vitro

• DOI: 10.1128/cvi.00022-09
• Publication Date: 2009-04-23T00:55:42Z
• Authors: Soad Nady; James Ignatz-Hoover; Mohamed T. Shata
• Keywords: Ex vivo; Interleukin 3

ABSTRACT Recently, a new lineage of CD4 + T cells in humans and mice has been reported. This helper cell secretes interleukin-17 (IL-17) defined as 17 (Th17). Th17 express the IL-23 receptor (IL-23R) play an important pathogenic role different inflammatory conditions. In this study, our aim was to characterize optimum conditions for isolation propagation human peripheral blood vitro clones. To isolate cells, two steps were taken. Initially, we negatively isolated from mononuclear normal donor. Then, IL-23R cells. Functional studies revealed that could be stimulated ex vivo with anti-CD3/CD28 secrete both IL-17 gamma interferon (IFN-γ). Furthermore, expanded 1 week presence anti-CD3/CD28, irradiated autologous feeder cytokines. Our data indicate cytokine treatment increased number propagated 14- 99-fold. evaluation cytokines all used (IL-2, IL-7, IL-12, IL-15, IL-23) amount IFN-γ secreted by at levels. results IL-7 plus IL-12 combination expansion secretion IFN-γ, while preferentially these predominately IL-17.