Random Sequencing of Paramecium Somatic DNA

• DOI: 10.1128/ec.1.3.341-352.2002
• Publication Date: 2002-12-05T01:42:23Z
• Authors: Linda Sperling; Philippe Dessen; Marek Zagulski; Ron E. Pearlman; Andrzey Migdalski; Robert Gromadka; Marine Froissard; Anne-Marie Keller; Jean Cohen
• Keywords: ORFS

ABSTRACT We report a random survey of 1 to 2% the somatic genome free-living ciliate Paramecium tetraurelia by single-run sequencing ends plasmid inserts. As in all ciliates, germ line (100 200 Mb) is reproducibly rearranged at each sexual cycle produce expressed or potentially genes, stripped repeated sequences, transposons, and AT-rich unique sequence elements limited line. found be compact (>68% coding, estimated from several complete library inserts) feature uniformly small introns (18 35 nucleotides). This facilitated gene discovery: 722 open reading frames (ORFs) were identified similarity with known proteins, 119 novel ORFs tentatively internal comparison data set. determined phylogenetic position respect eukaryotes whose genomes have been sequenced distance matrix neighbor-joining method using combined protein project. The unrooted tree obtained very robust excellent agreement accepted topology, providing strong support for quality consistency Our study demonstrates that good strategy discovery this organism.