Pho5p and Newly Identified Nucleotide Pyrophosphatases/ Phosphodiesterases Regulate Extracellular Nucleotide Phosphate Metabolism inSaccharomyces cerevisiae

• DOI: 10.1128/ec.4.11.1892-1901.2005
• Publication Date: 2005-11-08T23:18:02Z
• Authors: Eileen J. Kennedy; Lorraine Pillus; Gourisankar Ghosh
• Keywords: Pyrophosphatases; Physarum

ABSTRACT Extracellular nucleotides play many biological roles, including intercellular communication and modulation of nucleotide receptor signaling, are dependent on the phosphorylation state nucleotide. Regulation is necessary, a specialized class enzymes, pyrophosphatases/phosphodiesterases (E-NPPs), has been identified in mammals to perform this function. Although E-NPP conserved among complex eukaryotes, system not yet Saccharomyces cerevisiae. Using genetic biochemical experiments, we show that two orthologs family, referred as Npp1p Npp2p, exist budding yeast can phosphate hydrolysis. This activity enhanced during starvation, where hydrolyzed phosphates be imported from extracellular sources utilized overcome starvation through Pho5p acid phosphatase. The added compensatory effect by also newly established role for Pho5p. study demonstrates metabolism appears controlled at least independent regulatory mechanisms, uniting with regulation.