Galactomannoproteins of Aspergillus fumigatus

• DOI: 10.1128/ec.4.7.1308-1316.2005
• Publication Date: 2005-07-07T19:08:27Z
• Authors: W. Morelle; M. Bernard; J.-P. Debeaupuis; M. Buitrago; M. Tabouret; J.-P. Latgé
• Keywords: 0301 basic medicine; 6-Phytase; Antigens, Fungal; Membrane Glycoproteins; Aspergillus fumigatus; Enzyme-Linked Immunosorbent Assay; Sensitivity and Specificity; Gas Chromatography-Mass Spectrometry; Hydrofluoric Acid; 3. Good health; 03 medical and health sciences; Polysaccharides; alpha-Mannosidase; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Type C Phospholipases; Aspergillosis; Humans; Antibodies, Fungal

ABSTRACT Galactofuranose-containing molecules have been repeatedly shown to be important antigens among human fungal pathogens, including Aspergillus fumigatus . Immunogenic galactofuran determinants poorly characterized chemically, however. We reported here the characterization of two glycoproteins A. with an N-glycan containing galactofuranose. These proteins are a phospholipase C and phytase. Chemical indicates that it is mixture Hex 5-13 HexNAc 2 oligosaccharides, major molecular species corresponding 6-8 The contained one galactofuranose unit was in terminal nonreducing position attached Man. This single essential for immunoreactivity N-glycans assessed either monoclonal antibody recognizes tetra-β-1,5-galactofuran chain galactomannan or -infected patient sera.