C 4 -Dicarboxylate Utilization in Aerobic and Anaerobic Growth

• DOI: 10.1128/ecosalplus.esp-0021-2015
• Publication Date: 2016-11-05T12:45:12Z
• Authors: Gottfried Unden; Alexander Strecker; Alexandra Kleefeld; Ok Bin Kim
• Keywords: Fumarate reductase; Tartrate; Succinic acid; Fumarase; Cellular respiration; Microbial Metabolism

C4-dicarboxylates and the C4-dicarboxylic amino acid l-aspartate support aerobic anaerobic growth of Escherichia coli related bacteria. In growth, succinate, fumarate, D- L-malate, L-aspartate, L-tartrate are metabolized by citric cycle associated reactions. Because interruption under conditions, metabolism depends on fumarate reduction to succinate (fumarate respiration). some bacteria (e.g., Klebsiella), utilization C4-dicarboxylates, such as tartrate, is independent respiration uses a Na+-dependent membrane-bound oxaloacetate decarboxylase. Uptake into (and export succinate) achieved conditions different sets secondary transporters. Expression genes for C4-dicarboxylate induced in presence external DcuS-DcuR two-component system. Noncommon like l-tartrate or D-malate perceived cytoplasmic one-component sensors/transcriptional regulators. This article describes pathways their regulation. The cycle, respiration, reductase covered other articles discussed here only context metabolism. Recent aspects transport, sensing, regulation will be treated more detail. an updated version published 2004 EcoSal Plus. update includes new literature, but, particular, sections noncommon regulation, regulatory system, production engineered E. largely revised new.