Vitamin D-Mediated Induction of Innate Immunity in Gingival Epithelial Cells

• DOI: 10.1128/iai.00099-11
• Publication Date: 2011-03-22T04:46:16Z
• Authors: Laura McMahon; Kyell Schwartz; Ozlem Yilmaz; Eleith Brown; Lisa K. Ryan; Gill Diamond
• Keywords: 0301 basic medicine; Reverse Transcriptase Polymerase Chain Reaction; Immunoblotting; Gingiva; Fluorescent Antibody Technique; Epithelial Cells; Immunity, Innate; 3. Good health; 03 medical and health sciences; Gene Expression Regulation; Cathelicidins; Host-Pathogen Interactions; Humans; Receptors, Calcitriol; Vitamin D; Cells, Cultured; Antimicrobial Cationic Peptides

ABSTRACT Human gingival epithelial cells (GEC) produce peptides, such as β-defensins and the cathelicidin LL-37, that are both antimicrobial modulate innate immune response. In myeloid airway cells, active form of vitamin D 3 [1,25(OH) 2 ] increases expression antibacterial activity LL-37. To examine on defense epithelium, cultured were treated with either 10 −8 M 1,25(OH) or ethanol for up to 24 h. A time-dependent induction LL-37 mRNA 13-fold at h in standard monolayer three-dimensional cultures was observed. Induction receptor 1-α-hydroxylase genes also The hydroxylase functional, observed response stimulation by 25(OH)D . Through microarray analysis other genes, CD14 increased 4-fold, triggering expressed cells-1 (TREM-1) upregulated 16-fold after treatment TREM-1 is a pivotal amplifier macrophages, leading production inflammatory genes. Activation GEC led an increase interleukin-8 (IL-8) levels. Incubation against periodontal pathogen Aggregatibacter actinomycetemcomitans when bacteria added apical surface. This study first demonstrate effect oral suggesting could be utilized enhance cavity.