Staphylococcus aureus -Induced Corneal Inflammation Is Dependent on Toll-Like Receptor 2 and Myeloid Differentiation Factor 88

• DOI: 10.1128/iai.00645-06
• Publication Date: 2006-08-22T22:13:07Z
• Authors: Yan Sun; Amy G. Hise; Carolyn M. Kalsow; Eric Pearlman
• Keywords: Corneal inflammation

ABSTRACT Toll-like receptors (TLRs) expressed by the corneal epithelium represent a first line of host defense to microbial keratitis. The current study examined role TLR2, TLR4, and TLR9 common adaptor molecule myeloid differentiation factor 88 (MyD88) in Staphylococcus aureus model inflammation. epithelia C57BL/6, TLR2 −/− , TLR4 MyD88 mice were abraded using trephine epithelial brush exposed heat- or UV-inactivated S. clinical strain 8325-4 other isolates. Corneal thickness haze measured vivo confocal microscopy, neutrophil recruitment stroma was quantified immunohistochemistry, cytokine production enzyme-linked immunosorbent assay. exposure induced increased control C57BL/6 but not mice. responses similar those -induced cells neutrophils also significantly reduced compared with that These findings indicate inflammation is mediated resident infiltrating neutrophils.