Effects of ibeA Deletion on Virulence and Biofilm Formation of Avian Pathogenic Escherichia coli

• DOI: 10.1128/iai.00821-10
• Publication Date: 2010-10-26T04:14:26Z
• Authors: Shaohui Wang; Chunling Niu; Zhenyu Shi; Yongjie Xia; Muhammad Yaqoob; Jianjun Dai; Chengping Lu
• Keywords: Virulence factor; Strain (injury)

The ibeA gene is located on a genomic island, GimA, which involved in the pathogenesis of neonatal meningitis Escherichia coli (NMEC) and avian pathogenic E. (APEC). prevalence APEC collection China was investigated, 20 467 strains (4.3%) were positive. In addition, analysis association reference (ECOR) groups with positive revealed that linked to group B2. DE205B analyzed compared those NMEC, indicated specificity not consistent along pathotypes. invasion chicken embryo fibroblast DF-1 cells by RS218 observed, suggested could be model study mechanism invasion. inactivation led reduced capacity invade cells, defective virulence vivo, decreased biofilm formation wild-type strain. strain AAEC189 expressing exhibited enhanced formation. results quantitative real-time reverse transcription-PCR (qRT-PCR) animal system infection experiments loss colonization proliferation capacities brain during infection.