Adherent-Invasive Escherichia coli Production of Cellulose Influences Iron-Induced Bacterial Aggregation, Phagocytosis, and Induction of Colitis

• DOI: 10.1128/iai.00904-15
• Publication Date: 2015-07-28T01:01:53Z
• Authors: Melissa Ellermann; Eun Young Huh; Bo Liu; Ian M. Carroll; Rita Tamayo; R. Balfour Sartor
• Keywords: Proinflammatory cytokine; Bacterial Cellulose

Adherent-invasive Escherichia coli (AIEC), a functionally distinct subset of resident intestinal E. associated with Crohn's disease, is characterized by enhanced epithelial adhesion and invasion, survival within macrophages, biofilm formation. Environmental factors, such as iron, modulate production extracellular structures, which in turn influence the formation multicellular communities, biofilms, bacterial interactions host cells. However, physiological functional responses AIEC to variable iron availability have not been thoroughly investigated. We therefore impact on physiology strain NC101 subsequent macrophages. Iron promoted cellulose-dependent aggregation NC101. Bacterial cells recovered from aggregates were more susceptible phagocytosis than planktonic cells, corresponded decreased macrophage proinflammatory cytokine interleukin-12 (IL-12) p40. Prevention aggregate through disruption cellulose reduced iron-exposed In contrast, under iron-limiting conditions, where induced, secretion IL-12 Finally, abrogation induction colitis when was monoassociated inflammation-prone Il10(-/-) mice. Taken together, our results introduce novel factor that impacts host-microbe-environment alters potential AIEC.