Characterization of Early Gamma Interferon (IFN-γ) Expression during Murine Listeriosis: Identification of NK1.1+CD11c+Cells as the Primary IFN-γ-Expressing Cells

• DOI: 10.1128/iai.01026-06
• Publication Date: 2006-12-12T10:40:04Z
• Authors: Shu-Rung Chang; Kung-Jiun Wang; Yan-Feng Lu; Lii-Jia Yang; Wei-Jie Chen; Yu-Hsiang Lin; Hsin-Hou Chang; Shih-Lien Wang
• Keywords: CD11c

ABSTRACT Though it is well established that gamma interferon (IFN-γ) crucial to the early innate defense of murine listeriosis, its sources remain controversial. In this study, intracellular cytokine staining IFN-γ-expressing splenocytes after Listeria monocytogenes infection revealed NK1.1 + , CD11c CD8 T, and CD4 T cells expressed IFN-γ 24 h infection. Contrary previous report, most dendritic (DC) were CD8α − DC. Unexpectedly, almost all also NK1.1. These represented primary situ studies showed these recruited borders infectious foci IFN-γ. A significant population was found in uninfected spleen, lymph node, blood, bone marrow cells. And number increased significantly L. Using interleukin-12 (IL-12) p40 −/− mice, expression be largely IL-12 dependent, only about one-third wild-type mice. Moreover, absolutely dependent on live infection, as no detected inoculation heat-killed . Our findings not provide an insight into vivo but may change current perceptions DC natural killer