Intracellular Trafficking ofBordetella pertussisin Human Macrophages

• DOI: 10.1128/iai.01031-09
• Publication Date: 2010-01-12T02:44:53Z
• Authors: Yanina A. Lamberti; Jimena Alvarez Hayes; Maria L. Perez Vi...; Eric T. Harvill; Maria Eugenia Rod...
• Keywords: Intracellular parasite; Bordetella; Phagolysosome

Although Bordetella pertussis has been observed to survive inside macrophages, its ability resist or evade degradation in phagolysosomes not defined. We here investigated the trafficking of B. upon entry into human macrophages. During first hours following phagocytosis, a high percentage bacteria were destroyed within acidic compartments positive for lysosome-associated membrane proteins (LAMP). However, roughly one-fourth taken up this initial killing event, remaining nonacidic compartments. Forty-eight after infection, number intracellular per cell increased, suggesting that is capable replicating type compartment. Viable accumulated phagosomal early endosomal marker Rab5 but late LAMP. Moreover, pertussis-containing phagosomes acquired exogenously added transferrin, indicating have access extracellular components and essential nutrients via host recycling pathway. Overall, these results suggest survives eventually replicates with characteristics endosomes, potentially contributing extraordinary persist hosts populations.