Gene Models, Expression Repertoire, and Immune Response of Plasmodium vivax Reticulocyte Binding Proteins

• DOI: 10.1128/iai.01117-15
• Publication Date: 2015-12-29T03:23:25Z
• Authors: Jenni Hietanen; Anongruk Chim-ong; Thanprakorn Chira...; Jakub Gruszczyk; Wanlapa Roobsoong; Wai-Hong Tham; Jetsumon Sattabon...; Wang Nguitragool
• Keywords: Pseudogene; Reticulocyte

ABSTRACT Members of the Plasmodium vivax reticulocyte binding protein (PvRBP) family are believed to mediate specific invasion reticulocytes by P. . In this study, we performed molecular characterization genes encoding members family. Through cDNA sequencing, constructed full-length gene models and verified that coding those pseudogenes. We also used quantitative PCR measure their in vivo transcript abundances clinical isolates. Like related ligands falciparum , Pvrbp expression levels vary broadly across different parasite antibody measurements, found host immune pressure may be driving force behind distinctly high diversity one members, PvRBP2c. Mild yet significant negative correlation was between parasitemia PvRBP2b level, suggesting antibodies interfere with invasion.