A Novel ChimericPlasmodium vivaxCircumsporozoite Protein Induces Biologically Functional Antibodies That Recognize both VK210 and VK247 Sporozoites

• DOI: 10.1128/iai.01667-06
• Publication Date: 2006-12-12T10:40:04Z
• Authors: Anjali Yadava; Jetsumon Sattabon...; Michael A. Washin...; Lisa A. Ware; Victoria Majam; Hong Zheng; Sanjai Kumar; Christian F. Ocke...
• Keywords: Circumsporozoite protein; Infectivity; Plasmodium (life cycle)

A successful vaccine against Plasmodium vivax malaria would significantly improve the health and quality of lives more than 1 billion people around world. subunit is only option in absence long-term culture P. parasites. The circumsporozoite protein that covers surface sporozoites one best-studied malarial antigens most promising clinical trials. We report here development a novel "immunologically optimal" recombinant expressed Escherichia coli encodes chimeric CS encompassing repeats from two major alleles, VK210 VK247. This molecule widely recognized by sera patients naturally exposed to infection induces highly potent immune response genetically disparate strains mice. Antibodies immunized animals recognize both VK247 sporozoites. Furthermore, these antibodies appear be protective nature since they cause agglutination live sporozoites, an vitro surrogate sporozoite infectivity. These results strongly suggest biologically active immunogenic across histocompatibility complex raises prospect humans this may induce responses.