Cytolethal Distending Toxin ofHaemophilus ducreyiInduces Apoptotic Death of Jurkat T Cells

• DOI: 10.1128/iai.67.12.6394-6402.1999
• Publication Date: 2020-04-08T12:56:19Z
• Authors: Valentina Gelfanova; Eric J. Hansen; Stanley M. Spinola
• Keywords: Haemophilus ducreyi; Jurkat cells; Cytolethal distending toxin

ABSTRACT The immune response to Haemophilus ducreyi is mediated in part by T cells infiltrating the site of infection. In this study, we show that H. antigen preparations inhibited proliferation peripheral blood mononuclear and primary human T-cell lines. also Jurkat induced apoptosis cells, confirmed through detection DNA degradation membrane unpacking. cytotoxic product(s) was present cell-free culture supernatant whole-cell heat labile. produces two known heat-labile toxins, a hemolysin cytolethal distending toxin (CDT). Whole supernatants prepared from hemolysin-deficient mutant had same inhibitory apoptotic effects on as did its isogenic parent. Preparations made an cdtC were less toxic than activity restored complementation trans . CdtC-neutralizing antibodies -induced toxicity apoptosis. data suggest CDT may interfere with responses induction