Differential Protective Efficacy of DNA Vaccines Expressing Secreted Proteins ofMycobacterium tuberculosis

• DOI: 10.1128/iai.67.4.1702-1707.1999
• Publication Date: 2020-03-09T15:44:50Z
• Authors: Arun T. Kamath; Carl G. Feng; Murdo Macdonald; Helen Briscoe; Warwick J. Britton
• Keywords: Tuberculosis vaccines; BCG vaccine

ABSTRACT The development of more-effective antituberculosis vaccines would assist in the control global problem infection with Mycobacterium tuberculosis . One recently devised vaccination strategy is immunization DNA plasmids encoding individual microbial genes. Using genes for M. secreted proteins MPT64 (23 kDa), Ag85B (30 and ESAT-6 (6 kDa) as candidate antigens, were prepared tested immunogenicity protective efficacy a murine model aerosolized (TB). Intramuscular DNA-64 or DNA-85B resulted activation CD4 + T cells, which produce gamma interferon (IFN-γ), high titers specific immunoglobulin G antibodies. Further, induced major histocompatibility complex class I-restricted CD8 cytotoxic cells. addition eukaryotic leader sequence to mpt64 did not significantly increase T-cell antibody response. Each three vectors stimulated significant reduction level lungs mice challenged 4 weeks after immunization, but levels resulting bovis BCG. showed consistent hierarchy protection, most effective being Ag85B, followed by then MPT64. Coimmunization greater degree protection than that any single vector. This was associated emergence IFN-γ-secreting cells earlier infected animals immunized these suggests multisubunit may contribute future vaccine strategies against TB.