Saccharomyces boulardiiPreserves the Barrier Function and Modulates the Signal Transduction Pathway Induced in EnteropathogenicEscherichia coli-Infected T84 Cells

• DOI: 10.1128/iai.68.10.5998-6004.2000
• Publication Date: 2002-07-27T10:03:59Z
• Authors: Dorota Czerucka; Stephanie Dahan; Baharia Mograbi; Bernard Rossi; Patrick Rampal
• Keywords: Saccharomyces boulardii; Enteropathogenic Escherichia coli; Internalization

ABSTRACT Use of the nonpathogenic yeast Saccharomyces boulardii in treatment infectious diarrhea has attracted growing interest. The present study designed to investigate effect this on enteropathogenic Escherichia coli (EPEC)-associated disease demonstrates that S. abrogated alterations induced by an EPEC strain transepithelial resistance, [ 3 H]inulin flux, and ZO-1 distribution T84 cells. Moreover, EPEC-mediated apoptosis epithelial cells was delayed presence . did not modify number adherent bacteria but lowered 50% intracellular bacteria. Infection tyrosine phosphorylation several proteins cells, including p46 p52 SHC isoforms, attenuated Similarly, EPEC-induced activation ERK1/2 mitogen-activated protein (MAP) kinase pathway diminished yeast. Interestingly, inhibition with specific inhibitor PD 98059 decreased internalization, suggesting modulation MAP might account for lowering observed Altogether, demonstrated exerts a protective after adhesion modulating signaling bacterial infection.