Distribution of Core Oligosaccharide Types in Lipopolysaccharides from Escherichia coli

• DOI: 10.1128/iai.68.3.1116-1124.2000
• Publication Date: 2002-07-27T10:03:59Z
• Authors: Karen Amor; David E. Heinrichs; Emilisa Frirdich; Kim Ziebell; Roger P. Johnson; Chris Whitfield
• Keywords: STX2

ABSTRACT In the lipopolysaccharides of Escherichia coli there are five distinct core oligosaccharide (core OS) structures, designated K-12 and R1 to R4. The objective this work was determine prevalences these OS types within species. Unique sequences in waa biosynthesis) gene operon were used develop a PCR-based system that facilitated unequivocal determination isolates E. . This applied 72 ECOR collection, compilation is considered be broadly representative genetic diversity Fifty (69.4%) contained OS, 8 (11.1%) representatives R2, R3, 2 (2.8%) R4, only 4 (5.6%) K-12. type found all four major phylogenetic groups (A, B1, B2, D) collection. Virulent extraintestinal pathogenic tend closely related group B2 and, lesser extent, D isolates. All from had OS. contrast, commensal more A, which contains representing each structures. R3 38 verotoxigenic (VTEC) humans cattle belonging common enterohemorrhagic serogroups O157, O111, O26. Although other VTEC showed diversity, (83.1% isolates) still predominant. When non-VTEC analyzed, it most possessed type.