Intracellular Survival ofLeishmania majorin Neutrophil Granulocytes after Uptake in the Absence of Heat-Labile Serum Factors

• DOI: 10.1128/iai.70.2.826-835.2002
• Publication Date: 2002-07-27T09:55:53Z
• Authors: Helmut Laufs; Kerstin Müller; Jens Fleischer; Norbert Reiling; Nicole Jahnke; Jens C. Jensenius; Werner Solbach; Tamás Laskay
• Keywords: Kinetoplastida; Antibody opsonization; Intracellular parasite; Leishmania mexicana

ABSTRACT The role of polymorphonuclear neutrophil granulocytes (PMN) in defense against the intracellular parasite Leishmania is poorly understood. In present study, interaction human PMN with major promastigotes was investigated vitro. presence fresh serum, about 50% phagocytosed parasites within 10 min and uptake led to activation, resulting killing most ingested parasites. Heat inactivation serum markedly reduced rate early phagocytosis, suggesting a complement components . However, over were able ingest heat-inactivated if coincubation extended 3 h. After h, 10% found internalize even under serum-free conditions. These findings indicate that possess mechanisms for both opsonin/complement-dependent -independent Both pathways could be partially blocked by anti-CR3 antibody. Mannan-binding lectin not involved this process. When absence opsonin, majority survived intracellularly at least 1 day. data suggest dual response L. infection. On one hand, can rapidly eliminate parasites, on other survive PMN. data, together finding intact seen isolated from skin infected mice, serve as host cells survival first hours or days after