EnterohemorrhagicEscherichia coliInfection Induces Interleukin-8 Production via Activation of Mitogen-Activated Protein Kinases and the Transcription Factors NF-κB and AP-1 in T84 Cells

• DOI: 10.1128/iai.70.5.2304-2310.2002
• Publication Date: 2002-07-27T10:09:01Z
• Authors: Stephanie Dahan; Valere Busuttil; Veronique Imbert; Jean-Francois Peyron; Patrick Rampal; Dorota Czerucka
• Keywords: Proinflammatory cytokine; Interleukin 8

ABSTRACT Enterohemorrhagic Escherichia coli (EHEC) infections are associated with hemorrhagic colitis and the hemolytic-uremic syndrome (HUS). In vivo, elevated plasma levels of proinflammatory cytokine interleukin-8 (IL-8) in EHEC-infected children correlated a high risk developing HUS. As IL-8 gene transcription is regulated by factors NF-κB AP-1, we analyzed role these regulation production after infection epithelial intestinal T84 cell line EHEC. By 6 h infection, EHEC had induced significant secretion (35.84 ± 6.76 ng/ml versus 0.44 0.04 control cells). AP-1 activation 3 infection. Moreover, three mitogen-activated protein kinases (MAPK) (ERK1/2, p38, JNK) were phosphorylated cells concomitant induction DNA binding activity, IκB-α was then degraded activity. Pretreatment highly specific MEK1/2 inhibitor U0126, p38 SB203580, and/or proteasome ALLN led to inhibition cells. These findings demonstrate that (i) can induce vitro potent response (ii) due involvement MAPK, signaling pathways.