Characterization of Adenylate Cyclase-Hemolysin Gene Duplication in a Bordetella pertussis Isolate

• DOI: 10.1128/iai.72.8.4874-4877.2004
• Publication Date: 2004-07-23T02:05:05Z
• Authors: Karine Dalet; Christian Weber; Laurent Guillemot; Elisabeth Njamkepo; Nicole Guiso
• Keywords: 0303 health sciences; Whooping Cough; Macrophages; Bordetella pertussis; Cell Line; 3. Good health; Trachea; Disease Models, Animal; Mice; 03 medical and health sciences; Gene Duplication; Adenylate Cyclase Toxin; Animals; Humans

ABSTRACT

We describe a clinical isolate of Bordetella pertussis , the agent responsible for whooping cough, composed of at least two clones harboring one or two copies of the cya locus encoding one of the major toxins, adenylate cyclase-hemolysin. No difference was observed between the two clones in murine and cellular models, probably due to the high instability of the cya locus duplication.